2024-03-29T01:46:20Z
https://jmh.journals.ekb.eg/?_action=export&rf=summon&issue=219
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Mesenchymal Stem Cells Derived Extracellular Vesicles
Lamiaa
Abd ElFattah
Mesenchymal stem cells (MSCs) since their initial discovery have been a subject of intense investigations. Paracrine activity is the predominent mechanism of MSCs in tissue repair via the release of extracellular vesicles (EVs). Mesenchymal stem cells derived extracellular vesicles (MSCs-EVS) are small spherical membrane bounded fragments in the conditioned medium of MSCs. EVs differ in their origin, size and contents,yet they have the ability to induce changes in the recipient cells. Several studies were carried out investigating the therapeutic potentials of MSCs-EVs in many fields including cardiovascular, respiratory, renal diseases and cancer. They were also reported to have immunomodulatory effects. EVs have many advantages over the use of MSCs including the ability to home to target tissue with no organ accumulation, no toxicity and no long term maldifferentiation of engrafted cells. In addition, EVs are able to deliver biological materials safer than current methods including viral vehicles. MSCs-EVs are promising biomedical tools in stem cell based therapies.
Extracellular vesicles
cell based therapy
stem cells
2017
06
01
1
7
https://jmh.journals.ekb.eg/article_3765_3f930b3b094a138354ce2509985dc720.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Comparative Histological Study on the Effect of Stem Cells, and Gene Modified Stem Cells in Experimentally-Induced Diabetes Type 1 Cardiomyopathy
Sahar
Abo ElFadl
Maha
Shendi
Laila
Rashed
Ahmed
Reda
Background and Objectives: Sarco(endo) plasmic reticulum Ca2+-ATPase (SERCA2a) is the ATP-driven pump thattranslocates Ca2+ from the cytoplasm to the lumen of the sarcoplasmic reticulum. 25% of children and adolescents withtype 1 diabetes will develop diastolic dysfunction that results in part from a reduction in the activity of SERCA2a. Thepresent study aimed at investigating and comparing the therapeutic effect of Adipose Mesenchymal Stem Cells (AMSCs)with SERCA2a gene modified AMSCs in diabetes type 1 induced cardiomyopathy of adult male albino rat.Materials and Methods: Twenty-nine adult male albino rats were divided into: Donor group of 2 rats used to obtainAMSCs. Group I (Control group): 6 rats not exposed to diabetes induction. Group II (Diabetic group): 7 rats injectedwith streptozotocin (STZ) 50mg/Kg once. Group III ( (AMSCs group): 7 rats injected with AMSCs following inductionof diabetes. Group IV (SERCA2a Modified AMSCs Group): 7 rats injected with SERCA2a modified AMSCs, followinginduction of diabetes. All rats were sacrificed 8 weeks from start of experiment.Results: Morphological changes, indicating inflammation and degeneration, were found in the cardiac muscle of diabeticrats and regressed remarkably by AMSCs and SERCA2a modified AMSCs. The regression of morphological changes wasconfirmed by histological, immunohistochemical, morphometric and serological studies.Conclusion: The therapeutic effect of SERCA2a modified AMSCs in diabetes type 1 cardiomyopathy was moreremarkable than that of AMSCs.
Adipose Mesenchymal Stem Cells
cardiomyopathy
diabetes
SERCA2a
2017
06
04
8
18
https://jmh.journals.ekb.eg/article_3766_c8a1644352db568b181fcd7e67676e89.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Resveratrol pre- and post-treatment in doxorubicin-induced cardiac injury in relation to endogenous stem cell activation
Sahar
Abo Elfadl
Maha
Shendi
Ahmed
Reda
Aliaa
Abdelhaleem
Background: Doxorubicin (DOX), is a highly efficient anti-neoplastic drug used for treatment of solid tumors includingbreast cancer (BC). Resveratrol (RES) is considered an interesting molecule in hormone dependent cancer therapy. Stem cell activation may induce less cardiomyocyte apoptosis, enhanced angiogenesis and increased proliferation of cardiomyocytes. Materials and Methods: Twenty seven adult male albino rats, were divided into: control group including 6 rats, group II (DOX group) including 7 rats that received intraperitoneal (IP) injection with DOX 3 mg /kg 3 times a week for 2 weeks. Group III (RES post-treatment group) included 7 rats that were injected with DOX followed by 50 mg/Kg RES daily orally for another 2 weeks. Group IV (RES pretreatment group) included 7 rats that were injected with DOX and received concomitantly 50 mg/Kg RES daily orally for 2 weeks and left untreated for another 2 weeks. By the end of the 4th week all animals were sacrificed. Cardiac muscle specimens were subjected to histological, immunohistochemical, morphometric and serological studies. Results: Myocardial injury appeared as obvious congestion, apoptosis, degeneration, increased collagen fibers content,obvious caspase3 expression and minimal proliferating cell nuclear antigen (PCNA) +ve and CD44 +ve expression in DOX group. Regression of the previous changes was more remarkable in group IV than in group III except for PCNA and CD44 expression that was more obvious in both treatment groups.Conclusion: RES proved amelioration of DOX induced myocardial injury that was more noticeable in RES early administration. RES post-treatment might have been more efficient if longer duration was applied.
Doxorubicin
myocardium
Resveratrol
stem cells
2017
06
01
19
29
https://jmh.journals.ekb.eg/article_3767_da12afe81400d334ef44b5dede553f2c.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Effect of Orlistat on the pancreas of the female albino rat: Histological and Histochemical study
Soha
Abdelwahab
Azza
Ali
Ahmed
Mahmoud
Introduction: Orlistat is the only FDA approved drug for long term management of obesity. It reduces the absorption ofdietary fat by inhibiting lipase enzyme. Many cases of pancreatitis were clinically reported in people who used orlistat.Aim of The work:The aim of the present study is to shed a light on the possible deleterious effect of orlistat on thenormal pancreatic tissue, and the molecular mechanisms beyond these changes. Materials and Methods: Thirty six female albino rats were divided into 4 groups: Control Group I (a) without treatment and I (b) received only1 ml fish oil. Group II received 30 mg/kg/day of orlistat dissolved in 1ml of fish oil orally for 8 weeks. Group III, received 40 mg/kg/day of orlistat .Group IV (a) & (b) withdrawal groups. Pancreas was dissected out and processed for histological and immunohistochemical study for CASPASE, TNF, INOS and INSULIN. Results: Orlistat caused variable degrees of pancreatic tissue degeneration, dilatation of pancreatic ducts with retained secretion, fatty degeneration and congestion. Inflammatory cells infiltration observed in both pancreatic acini and islets of Langerhan,s only with high dose (40mg/kg).Positive immunoreactivity for activated caspase 3 and iNOS being more evident in high dose and decrease inimmunoreactivity for anti-insulin antibody. immunoreactivity for TNF-α had been shown in high dose. These effectsdisappear after low dose withdrawal only. Conclusion: Orlistat may induce inflammation, fatty degeneration and impaired insulin production in the pancreas. These effects are dose dependent, and it should be taken in consideration while prescribing orlistat as a treatment for obesity.
Acini
ducts
Orlistat
pancreas
2017
06
01
30
43
https://jmh.journals.ekb.eg/article_3768_43577c04da1b84ebd812778f00741de7.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Light and electron microscopic study on the effect of immobilization stress on adrenal cortex of adult rats and possible ameliorative role of vitamin E.
Zainab
Altayeb
Maysara
Salem
Background: Modern society is full of stress. Immobilization stress is a model used in animals to study its effects. Vitamin E is an antioxidant that protects biological membranes from oxidative stress. Objectives: This study aimed to investigate the effects of immobilization stress on the adrenal cortex of rats and the ameliorative effect of vitamin E.Materials and Methods: Forty adult male rats were used in this study and were divided equally into 4 groups. Group I:were divided equally into negative and positive controls. Group II: rats received vitamin E at a dose of 40 I.U/kg by gastric tube for 30 days. Group III: rats subjected to immobilization stress 2 hours /day for 30 days. Group IV: rats subjected to immobilization stress 2 hours /day and received 40 I.U/kg of vitamin E for 30 days. Adrenal sections were histologically prepared and examined. Results: Group II was comparable to group IA. Group III revealed zona glomerulosa (ZG) with loss of normal architecture, zona fasciculata (ZF) with multiple cells containing cytoplasmic vacuolations and darkly stained nuclei. By EM ZG cells appeared with accumulations of lipid droplets and lysosomes, irregular nuclear envelopes and chromatin condensation. ZF cells showed numerous lipid droplets and irregular nuclear envelopes. There were significant increases in the mean area of collagen fibers and mean serum cortisol level. Group IV revealed regression of these changes and increase in the mean count of CD44 +ve cells.Conclusions: Immobilization stress exerted deleterious suprarenal cortical changes and vitamin E had an ameliorative effect.
adrenal cortex
Immobilization stress
suprarenal gland
Vitamin E
2017
06
01
44
56
https://jmh.journals.ekb.eg/article_3769_5a6076affed7de820e75069718a13993.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Role of vitamin A in the healing process of alkali caused corneal injury of adult male albino rat: Histological and immunohistochemical study
Soha
Abdelwahab
Nalaa
Abd El-Hameed
Entesar
Saber
Ahmed
Sayed
Background and Aim: Alkali burn of the cornea is considered as the most dangerous injury to the eye. It causes cornealinfection, ulceration, perforation, neovascularization (NV) and opacification. Vitamin A is necessary for the normal growth and differentiation of epithelium; it is expected to promote the mechanical repair of corneal epithelial defectsThe present study is planned to investigate the effect of vitamin A eyegel on wound healing of the corneal alkali burn in rats. Materials and Methods: A total of thirty male albino rats were used. Rats were divided randomly into four groups: Group I,the control group; included three rats, received distilled water. Each of group II, III, IV included 9 rats: their central corneas of the right eyes were injured by contacting them with filter paper saturated with 0.01 m NaOH for 45 seconds. Group II, the non-treated group, injured and received distilled water. Group III, the antibiotic treated group, received antibiotic eye drops (lincomycin hydrochloride eye drops) 3 times per day for 3 days. Group IV, vitamin A treated group, received the same course of antibiotic and vitamin A eye gel (Hypotear gel 1000 IU/g) three times per day. for 3 days. The eyeball was taken out, rapidly fixed and processed for light microscopic, immunohistochemical and morphometric studies. Specimens were taken after 24,48 and 72 hr. post-injury for tissue preparation and study of structural changes and immuno-histochemical analysis using Ki67 (detection of cellular proliferation) and transforming growth factor – beta (TGF-β) which is one of the most critical growth factors in establishing the pathologic lesion after corneal alkali burn. Results: Hematoxylin and eosin stained sections showed rapid healing of corneal ulcer in vitamin A treated group, with absence of neo-vasculariztion and inflammatory cellular infiltration. Immunohistochemical results showed that the use of vitamin A enhance cell proliferation (detected with Ki67) and decrease the expression of TGF-β which is one of the most critical growth factors in establishing the pathological lesion after corneal alkali burn.Conclusions: Vitamin A eye gel helped rapid healing of corneal alkali burn. This effect may be due to its anti-inflammatoryeffect and stimulation of cell proliferation.
alkali burn
cornea
vitamin A
2017
06
01
57
68
https://jmh.journals.ekb.eg/article_3770_4e7a5110fd419ca70a2bbd7cb49006ab.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Histological and immunohistochemical study of the role of stem cells, conditioned medium and microvesicles in treatment of experimentally induced acute kidney injury in rats
Maysara
Salem
Omayma
Helal
Hala
Metwaly
Asmaa
El Hady
Shaimaa
Ahmed
Background: Acute kidney injury (AKI) is a major health problem associated with high morbidity and mortality rates.Mesenchymal stem cells (MSCs) have revealed advantages for therapeutic use in medical practice. Microvesicles (MVs)are membranous, cell-derived vesicles released by MSCs into their microenvironment. The conditioned medium (CM) isthe medium surrounding MSCs. Aim of the Work: This study aimed to investigate the therapeutic potential of MSCs, their CM and microvesicles (MVs) on experimentally induced acute kidney injury in rats. Materials and Methods: Fifty-five rats were divided into five groups: Group I (control group). Group II: given glycerol intramuscularly. Group III: given glycerol then MSCs. Group IV: given glycerol then CM. Group V: given glycerol then MVs. Kidney specimens were processed for H&E and Ki-67 staining and EM studies. Results: Subgroup IIA revealed vacuolation of the cytoplasm, flattening of the epithelial lining the tubules, extrusion of cytoplasm and nuclei into luminal spaces, deeply stained nuclei, and hyaline material in tubular lumina. EM examination of proximal and distal convoluted tubules showed multiple vacuoles, lysosomes, loss of continuity of apical cell membrane,presence of debris in the lumina and vacuolated mitochondria. Group IIB revealed poor improvement with persistence of most lesions. Groups III, VI and V showed amelioration of most of these lesions, and decrease in blood urea and serum creatinine levels. Conclusion: Mesenchymal stem cells, CM and MVs ameliorate induced AKI and with little differences in their effectiveness. CM and MVs can be used in treating diseases.
Acute kidney injury
conditioned medium
glycerol
Ki-67
Mesenchymal Stem Cells
Microvesicles
2017
06
01
69
83
https://jmh.journals.ekb.eg/article_3771_d7fa5b05beb45cf4f47593932dff84d8.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
A possible mechanism of the protective effect of simvastatin on streptozotocin induced diabetic nephropathy in adult male rats (ahistological study)
Alshaymaa
Aboulkhair
Marwa
sabry
Ahmed
Rabiee
Background and Objectives: Diabetic nephropathy (DN) is the leading cause of end stage renal disease. The aim of thisstudy is to demonstrate the possible mechanism of reno-protective role of Simvastatin (SMV), a lipophilic compoundagainst streptozotocin induced diabetic nephropathy.Methods and Results: Thirty-five adult male albino rats were divided into control group, diabetes group and SMV treated diabetic group 40 mg/Kg/ day for 8 weeks. Animals were sacrificed and the kidneys were dissected out and prepared to be stained with hematoxylin and eosin, periodic acid sheif (PAS), toluidine blue and immunohistochemical staining against inducible nitric oxide synthase (iNOS). Ultrathin sections were also prepared. Morphometric measurements and statistical analysis were done. Diabetic group revealed glomerular and tubular affection in addition to significantly increased PAS staining and immunostaining of iNOS. Electron microscopy revealed affected glomerular basement membrane,endothelium and podocyte. Treatment with SMV markedly attenuated these changes with significant decrease in PAS stain and iNOS immunostain. Conclusion: Simvastatin could attenuate streptozotocin induced DN through many factors as lipo-protection, antiinflammatory and antioxidant effects. This anti-inflammatory and antioxidant effects could be through inhibition of iNOS enzyme and secondary decreased production of NO.
diabetic nephropathy
simvastatin (SMV)
Streptozotocin (STZ)
Ultrastructure
2017
06
01
84
96
https://jmh.journals.ekb.eg/article_3772_13347b39c60d699aa734a289ec925550.pdf
Journal of Medical Histology
2536-9172
2536-9172
2017
1
1
Role of Erythropoietin Induced Stem Cell Mobilization in the Repairing of Acute Kidney Injury Following Ischemia Reperfusion in Albino Rats: A Histological Study
Samir
Nada
Nagwa
Ahmed
Eman
Farag
Rokia
Hassan
Background: The incidence of acute kidney injury (AKI), the new term for acute renal failure, has increased in recentyears. Ischemia–reperfusion (IR) is one of the main causes of AKI. Erythropoietin (EPO) is a complex molecule, whichregulates red blood cell production in the bone marrow. Recent studies provide evidence of potential therapeutic effect ofEPO on AKI. Aim of the Work: Investigate the role of EPO in mobilization of bone marrow derived stem cells and its role in repairing AKI. Materials and Methods: This study included 32 adult male albino rats, divided into three groups; control, non-treated IR and EPO treated group. AKI was done by induction of IR via clamping both renal pedicles for 40 minutes and EPO 5000 U|kg was injected in treated group once intraperitoneally immediately after IR. Rats were sacrificed 48 hours and 1 week after intervention, then renal sections were stained with hematoxylin & eosin, PAS and immunohistochemically for CD34. Results: Erythropoietin improved the impaired kidney manifestations occurred by IR including increasing the reduced urea and creatinine levels, improved histological architecture of kidney. Also, increasing CD34 immunopositive hematopoietic and endothelial progenitor stem cells. Conclusion: This work concluded that administration of EPO at time of renal IR resulted in enhanced mobilization of stem cells resulting in structural recovery and improved functions.
Acute kidney injury
CD34
EPCs ischemia reperfusion
erythropoietin
hematopoietic stem cells
2017
06
06
97
109
https://jmh.journals.ekb.eg/article_3773_7e3b9dcd6fbc12f6b3be556e47f6353a.pdf