Cairo University, Faculty of MedicineJournal of Medical Histology2536-91725120210601Effect of Erythropoietin on Cisplatin Induced Nephrotoxicity in Adult Male Albino Rat: Histological, Ultrastructural and Biochemical Study12424669510.21608/jmh.2019.16381.1066ENHossam EmamDepartment of Anatomy, Faculty of Medicine, Cairo University, EgyptJournal Article20190901Introduction: Cisplatin is documented as an effective chemotherapeutic drug with many adverse effects on vital organs,<br />including nephrotoxicity, neurotoxicity and ototoxicity. Erythropoietin is a glycoprotein hormone synthesized by the renal<br />fibroblasts in the interstitial tissue of the renal cortex in response to hypoxia. Recent research work points out that it could to<br />improve the renal dysfunction and histopathological alterations caused by oxidative stress.<br />Aim: Is to examine the possible protective role of erythropoietin in cases with cisplatin induced nephrotoxicity.<br />Materials and Methods: The present study used fifty adult male albino rats, with their weights within the range of 180-220<br />g. The rats were divided into five groups, 10 rats each: Group I (Control group): Each of the rats in this group received a<br />single intraperitoneal injection of a normal saline solution (0.9% sodium chloride). Group II (Cisplatin administration group):<br />Each of the rats in this group was injected once with cisplatin intaperitoneally at a dose of 6 mg/kg. Group III (Cisplatin<br />and erythropoietin pre-administration group): Rats of this group were injected with a single dose of erythropoietin; 3000 IU/<br />kg intaperitoneally, and one day later they were injected with 6 mg/kg of cisplatin intaperitoneally. Group IV (Cisplatin and<br />erythropoietin co-administration group): Rats of this group were injected with a single dose of erythropoietin; 3000 IU/kg<br />intaperitoneally together with 6 mg/kg of cisplatin by the same rout. Group V (Erythropoietin and cisplatin post-administration<br />group): Rats of this group were injected with a single dose of 3000 IU/kg erythropoietin intaperitoneally, and five days later<br />they were given 6 mg/kg of cisplatin by the same rout.<br />Results: Cisplatin injection exerted various histopathological and laboratory alterations such as renal tubular cell necrosis,<br />nuclear pyknosis and cytoplasmic vacuolation as well as marked elevation of levels of blood urea and serum creatinine.<br />Erythropoietin could improve all the histopathological and functional deterioration exerted by cisplatin injection especially<br />when given prior to cisplatin administration.<br />Conclusion: Erythropoietin could prevent the adverse histological and functional alterations induced by intraperitoneal<br />injection of cisplatin in the kidney of adult male albino rat.https://jmh.journals.ekb.eg/article_246695_6adff13876f83d5d9e97a1d8303c407d.pdfCairo University, Faculty of MedicineJournal of Medical Histology2536-91725120210601Histological Study on the Effect of Bone Marrow Derived Mesenchymal Stem Cells Versus their Conditioned Medium and Microvesicles on Experimentally Induced Acute Cerebral Ischemia in Albino Rats254024669910.21608/jmh.2021.71729.1089ENSafinaz SayedDepartments of Histology, Faculty of Medicine, Cairo University,Sameh ElsonbatyFaculty of Medical and Health Sciences-Emirates College of Technology.Ghada IbrahimDepartments of Histology, Faculty of Medicine, October 6 University,
Egypt,Manal Abdel MohsenDepartments of Histology, Faculty of Medicine, Cairo University,0000-0002-8032-7454Journal Article20210409Background and Objectives: Ischemic stroke (an acute cerebrovascular event) is major health problem. It is associated with<br />high rates of mortality and long-term disabilities. The therapeutic options remain limited. This work aimed to evaluate and<br />compare the possible protective effect of bone marrow-derived mesenchymal stem cells (BM-MSCs), their conditioned media<br />(BM-MSCs-CM) and their derived microvesicles (BM-MSCs-MVs) on acute cerebral ischemia.<br />Materials and Methods: Fifty-two adult male albino rats were divided into five groups: Group I, Group II, Group III, Group < br />IV and Group V. Acute cerebral ischemia was induced in all experimental groups via transient right common carotid artery<br />(RCCA) occlusion. Brain specimens were processed for hematoxylin and eosin, immunohistochemical staining for glial<br />fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF). The mean area percent for both GFAP and<br />VEGF were measured and statically analyzed.<br />Results: RCCA occlusion resulted in morphological alterations in cerebral cortex of Group II. Significant increase in mean<br />area % of GFAP positive immunoreactivity and significant decrease in mean area % of VEGF positive immunoreactivity was<br />detected in this group as compared to Group I. Instead, the treated groups revealed amelioration in these changes. Significant<br />decrease in mean area % of GFAP positive immunoreactivity along with significant rise in mean area % of VEGF positive<br />immunoreactivity were detected in group V compared to other treated groups.<br />Conclusion: Administration of BM-MSCs-MVs recorded marked ameliorating effect in comparison to BM-MSCs and BMMSCs-<br />CM. Hence, it could be considered an ideal candidate as therapeutic agent in ischemic stroke.https://jmh.journals.ekb.eg/article_246699_0a1a082eaf29c12526d913318274b538.pdfCairo University, Faculty of MedicineJournal of Medical Histology2536-91725120210601The Possible Neurodegeneration of the CA3 and CA1 of Hippocampus in STZ - Induced Diabetes Mellitus Type1 on Male Wistar Rats414724670010.21608/jmh.2021.80629.1090ENShahriar AhmadpourDepartment of Anatomical Sciences, Medicine School, North Khorasan University of Medical
Sciences, Bojnurd, Iran.Khadijah FoghiDepartment of Anatomical Sciences, Medicine School, North Khorasan University of Medical
Sciences, Bojnurd, Iran.Journal Article20210614Introduction: Hippocampus is one of the most sensitive region of central nervous system to metabolic disorders. Structurally<br />it is composed of cornu ammonis and dentate gyrus. CA3 and CA1 play critical role in cognitive performance and psychosis.<br />Aim: The aim of this study was to study the effects of diabetes mellitus on bilateral CA3 and CA1.<br />Materials and Methods: Male adult wistar rats were randomly into two groups as diabetic and control. Diabetes mellitus was<br />induced by a single intraperitoneal (IP) injection of STZ at a dose of 60 mg/kg. The control animals only received saline. Two<br />months after uncontrolled diabetes, the animals were anesthetized. The harvested whole brains were stained with cresyl violet.<br />Results: The number of degenerated neurons in right side CA3 (600±25) showed significant level of difference with left CA3<br />(0) (P<0.001). The count of degenerated neurons in right side CA1 (90±5) showed significant difference with left side CA1<br />region (5±2) (P<0.001).<br />Conclusion: Our study showed that experimental diabetes mellitus type 1 leads to unilateral neurodegeneration in pyramidal<br />layer of CA3 and CA1 regions of right hippocampus.https://jmh.journals.ekb.eg/article_246700_b4764d5da04c021d97874c7b0016c584.pdfCairo University, Faculty of MedicineJournal of Medical Histology2536-91725120210601The Prophylactic Effect of Royal Jelly in Amelioration of 6-Mercaptopurine - Induced Testicular Damage in Albino Rats (Histological and Immunohistochemical study)485924670110.21608/jmh.2022.125328.1096ENSamraa Abdel-KawiDepartment of Medical Histology and Cell Biology, Faculty of Medicine, Beni-Suef University,
Egypt.Journal Article20220304Background: Testicular toxicity is a well-documented adverse impact of a large number of chemotherapeutic drugs.<br />6-mercaptopurine (6MP) is an anti-cancer agent with strong anti-tumor activity. Despite this, it has a detrimental impact on the<br />male reproductive system. Royal jelly (RJ) is a nutrient-dense substance that may also include antibacterial, antioxidant, and<br />anti-inflammatory characteristics. These qualities may account for the majority of royal jelly's health claims. This study aimed<br />to investigate the protective effect of RJ on 6MP-induced testicular damage in adult albino rats.<br />Materials and Methods: Forty male adult albino rats were categorized into four equivalent groups (n= 10): control, RJ group < br />(200 mg/kg RJ for 30 days), 6MP group (5 mg/kg 6MP for 20 days), and RJ+6MP group (200 mg/kg RJ for 10 days alone<br />then concomitant with 5 mg/kg 6MP for 20 days). Histological and immunohistochemical examinations of the testes were<br />performed.<br />Results: The histological results revealed that oral administration of 6MP triggers a substantial reduction in testis weights,<br />count, sperm motility %, and viability. 6MP also caused severe testicular degeneration with weak immunoreactivity to Bcl-<br />2and N-Cadherin. RJ attenuated testicular degeneration and upregulated the expression of Bcl-2 and N-Cadherin.<br />Conclusion: RJ reduced all testicular changes induced by 6MP. Thus, RJ may be a viable medication for infertility problems<br />associated with testicular injuries.https://jmh.journals.ekb.eg/article_246701_03b7f57e759a02d2da85b4d3ff8a09bc.pdfCairo University, Faculty of MedicineJournal of Medical Histology2536-91725120210601Histological and Immunohistochemical Study on the Effect of 900 MHz Mobile Phone Radiation on the Adrenal Cortex of the Adult Male Albino Rat and the Possible Protective role of Green Tea Extract607424670510.21608/jmh.2021.85297.1092ENHanaa MousaDepartment of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University,
EgyptJournal Article20210711Background and Objectives: Mobile phones emit electromagnetic waves that have adverse effects on many organs. Adrenal<br />cortex is an important gland in the human body. In this study, the effect of mobile phone radiation on the adrenal cortex and<br />the possible protective role of green tea extract were investigated.<br />Materials and Methods: Twenty- seven adult male Wistar albino rats were used. They were divided into three groups; group < br />I [the control group], group II [the mobile phone group], and group III [the mobile phone group received green tea extract<br />(GTE)]. Serum adrenocorticotrophic hormone (ACTH), and corticosterone and tissue malondialdehyde (MDA), catalase<br />(CAT) and superoxide dismutase (SOD) were measured. Gene expression of NF-κB was measured. Histological examination<br />was done using H&E, Masson trichrome, and caspase 3. Morphometric and statistical analyses were performed.<br />Results: Adrenal MDA was elevated and CAT and SOD were decreased in group II, while in group III significantly decreased<br />MDA and increased the activities of CAT and SOD. NF-κB was highly increased in group II and, moderately increased in<br />group III. Histological examination showed distortion of histological architecture, congestion and increased vacuolation ,<br />a significant increase in collagen fibers and caspase 3 immunoreaction in group II. In group III, there were restoration of<br />histological architecture and few areas of congestion and vacuolation and moderate increase in collagen fibers and caspase 3<br />immunoreaction.<br />Conclusions: Mobile phone radiation caused adverse effects on the function and structure of the adrenal cortex and the intake<br />of green tea significantly prevented this effect.https://jmh.journals.ekb.eg/article_246705_1501c0b83de4896d4a6b51f9dfd6a2db.pdfCairo University, Faculty of MedicineJournal of Medical Histology2536-91725120210601Histological and Immunohistochemical Study on the Effect of the Combined Vitamin C and Metformin versus Metformin Alone in Treating Diabetic Myopathy in Rat758724670610.21608/jmh.2021.98961.1093ENHanaa MousaDepartment of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University,
EgyptJournal Article20211001Background: Metformin is used in type 2 diabetes mellitus (T2DM). Vitamin C is a water-soluble vitamin with antioxidant and<br />anti-inflammatory properties. Therefore, the effect of combining metformin and vitamin C in treating the diabetic myopathy<br />was investigated in this research.<br />Methods: Forty rats were used divided into 4 groups; control group (group I), diabetic group (group II), metformin group < br />(group III), and vitamin C and metformin group (group IV). Body weight was measured before and at the end of the experiment.<br />Blood glucose levels and serum C-reactive protein (CRP) were measured. Tissue malondialdehyde (MDA) and superoxide<br />dismutase (SOD) were measured. Sections of the skeletal muscle were stained with H&E, Masson trichrome, and caspase-3 and<br />inducible nitric oxide synthase (iNOS) immnuostaining. Morphometric and statistical analyses for the results were performed.<br />Results: Body weight of rats of group I and group II were decreased then increased in group IV. Blood glucose levels were<br />elevated in group II; and reduced in group III and group IV. MDA level were increased in group II, then lowered in both in<br />group III and group IV.SOD activity was decreased in group II, then increased in group III and group IV toward normal level.<br />Collagen deposition and immune reaction to caspase-3 were elevated in group II and group III and group IV. Immune reaction<br />to iNOS was elevated in group II and group III, and nearly the same as group I in group IV.<br />Conclusions: Combined intake of vitamin C and metformin provided better results in treating diabetic myopathy than that<br />obtained from metformin alone.https://jmh.journals.ekb.eg/article_246706_2105f09f142ba5987c50b5be7bd50d07.pdfCairo University, Faculty of MedicineJournal of Medical Histology2536-91725120210601Nanocurcumin: A Promising Therapeutic Supplements Lessening Cadmium-Induced Lung Injury Of Adult Male Albino Rat. A Histopathological, Biochemical And Ultra-structural Study889224670910.21608/jmh.2022.133055.1098ENAhmed BayoumiDepartment of Anatomy and Embryology, Faculty of Medicine, Cairo University, Egypt0000-0002-6517-4234Hanan YassaDepartment of Anatomy and Embryology, Faculty of Medicine, Cairo University, EgyptRadwa MohamedDepartment of Anatomy and Embryology, Faculty of Medicine, Cairo University, EgyptMohamed El-sebaieDepartment of Anatomy and Embryology, Faculty of Medicine, Cairo University, EgyptAli MousaDepartment of Anatomy and Embryology, Faculty of Medicine, Cairo University, EgyptDoaa HassanDepartment of Anatomy and Embryology, Faculty of Medicine, Cairo University, EgyptJournal Article20210413Background: Cadmium (Cd) is considered a health hazard due to its industrial use. Lungs and kidneys are the primary organs<br />affected by its toxicity. Inflammatory response in lung tissue is mediated by Cd exposure until it becomes dysfunctional. Cd<br />damages DNA indirectly by increasing reactive oxygen species generation, which interacts with DNA defense system leading<br />to genomic instability. Curcumin (C) has multiple health benefits, as well as its Nano-formulation, Nano-curcumin (NC),<br />which has potential to increase biological and pharmacological actions of curcumin.<br />Aim: To focus on Cd-mediated inflammatory response and cell apoptosis in lung tissue of adult male albino rat and use NC as<br />a potential therapeutic agent.<br />Methods and methods: Thirty adult albino male rats were split into three groups, each with ten rats: control (group I), Cd<br />treated (group II), and Cd+NC treated (group III). Rats were sacrificed and lungs were extracted for light microscopic and<br />ultra-structural examination. IL-6 and TNF-α concentration were estimated and lung cellular survival genes; Bax and Bcl2<br />expression was evaluated by PCR.<br />Results: Alveolar damage with loss of normal lung architecture were seen in H&E-stained sections of group II confirmed by<br />ultrastructural investigation revealing cellular cytoplasmic vacuolations with degenerated mitochondria. In group II, IL-6,<br />TNF-α and Bax gene were increased, whereas Bcl2 gene was decreased with increased Bax/Bcl-2 genes ratio expression.<br />NC treated group showed cellular restoration, decreased pro-inflammatory cytokines and decreased Bax/Bcl-2 genes ratio<br />expression.<br />Conclusions: NC can be taken as a medicinal supplement to ease Cd poisoning risk in lungs.https://jmh.journals.ekb.eg/article_246709_bc3ae62cbd3c89b5be731bf6398fd183.pdf