Elsyade, R., Abdel Fattah, M., Khaled, D., Eldosoki, D. (2021). The Beneficial Effects of Lipoic Acid Versus Coenzyme Q10 on Arginine-Induced Acute Pancreatitis in Albino Rats: Histological and Immunohistochemical study. Journal of Medical Histology, 5(2), 154-170. doi: 10.21608/jmh.2022.161806.1103
Rania Elsyade; Marwa Abdel Fattah; Doaa Khaled; Doaa Eldosoki. "The Beneficial Effects of Lipoic Acid Versus Coenzyme Q10 on Arginine-Induced Acute Pancreatitis in Albino Rats: Histological and Immunohistochemical study". Journal of Medical Histology, 5, 2, 2021, 154-170. doi: 10.21608/jmh.2022.161806.1103
Elsyade, R., Abdel Fattah, M., Khaled, D., Eldosoki, D. (2021). 'The Beneficial Effects of Lipoic Acid Versus Coenzyme Q10 on Arginine-Induced Acute Pancreatitis in Albino Rats: Histological and Immunohistochemical study', Journal of Medical Histology, 5(2), pp. 154-170. doi: 10.21608/jmh.2022.161806.1103
Elsyade, R., Abdel Fattah, M., Khaled, D., Eldosoki, D. The Beneficial Effects of Lipoic Acid Versus Coenzyme Q10 on Arginine-Induced Acute Pancreatitis in Albino Rats: Histological and Immunohistochemical study. Journal of Medical Histology, 2021; 5(2): 154-170. doi: 10.21608/jmh.2022.161806.1103
The Beneficial Effects of Lipoic Acid Versus Coenzyme Q10 on Arginine-Induced Acute Pancreatitis in Albino Rats: Histological and Immunohistochemical study
1Department of Anatomy and Embryology, Faculty of Medicine, Helwan University,
2Department of Histology and Cytology, Misr University for Science and Technology
3Department of Histology and Cytology, Faculty of Medicine, Helwan University
4Department of Histology and Cytology, Faculty of Medicine, Fayoum University, Egypt.
Abstract
Background: Acute pancreatitis (AP) is a self-limiting condition to a widespread, deadly multi-organ failure that can result in death. Objectives: To compare the protective effect of alpha lipoic acid (ALA) and Coenzyme (Q10) in an experimental rat model of L-arginine-induced AP. Materials (Subjects): Forty adult male albino rats were randomized equally into 4 groups: (i) The control group; (ii) The AP group received L-Arginine by intraperitoneal (IP) injection in two doses of 2 g/kg body weight each, 1 hour apart, (iii) The ALA+AP group was injected with L-Arginine and intramuscular (IM) injection of ALA in two doses of 60mg/kg body weight; 1 hour before L-arginine injection and 24 hours after the second dose of L-arginine; (iii) The Coenzyme Q10+AP group was injected with L-Arginine and IM injection of Q10 in two doses of 30mg/kg body weight; 1 hour before L-arginine injection and 24 hours after the second dose of L-arginine. Histological, immunohistochemical for HSP70 and NF-κB, and morphometric studies were done followed by statistical analysis. Results: AP group showed significant distortion of the pancreatic acini with obvious cellular damage while those treated by ALA and Coenzyme Q 10 especially ALA showed significant pancreatic tissue improvement as shown by HSP70 and NF-κB immunoexpressing. Conclusions: ALA and Q 10 therapy proved a clear protective effect in suppressing experimentally generated AP in male albino rats, but the improvement was more obvious with ALA.
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