Abo ElFadl, S., Shendi, M., Rashed, L., Reda, A. (2017). Comparative Histological Study on the Effect of Stem Cells, and Gene Modified Stem Cells in Experimentally-Induced Diabetes Type 1 Cardiomyopathy. Journal of Medical Histology, 1(1), 8-18. doi: 10.21608/jmh.2017.1038.1015
Sahar Abo ElFadl; Maha Shendi; Laila Rashed; Ahmed Reda. "Comparative Histological Study on the Effect of Stem Cells, and Gene Modified Stem Cells in Experimentally-Induced Diabetes Type 1 Cardiomyopathy". Journal of Medical Histology, 1, 1, 2017, 8-18. doi: 10.21608/jmh.2017.1038.1015
Abo ElFadl, S., Shendi, M., Rashed, L., Reda, A. (2017). 'Comparative Histological Study on the Effect of Stem Cells, and Gene Modified Stem Cells in Experimentally-Induced Diabetes Type 1 Cardiomyopathy', Journal of Medical Histology, 1(1), pp. 8-18. doi: 10.21608/jmh.2017.1038.1015
Abo ElFadl, S., Shendi, M., Rashed, L., Reda, A. Comparative Histological Study on the Effect of Stem Cells, and Gene Modified Stem Cells in Experimentally-Induced Diabetes Type 1 Cardiomyopathy. Journal of Medical Histology, 2017; 1(1): 8-18. doi: 10.21608/jmh.2017.1038.1015
Comparative Histological Study on the Effect of Stem Cells, and Gene Modified Stem Cells in Experimentally-Induced Diabetes Type 1 Cardiomyopathy
1Departments of Medical Histology and Cell Biology , Faculty of Medicine, Cairo University, Cairo, Egypt.
2Departments of Biochemistry, Faculty of Medicine,Cairo University, Cairo, Egypt.
3Faculty of Pharmacy, Near East University, North Cyprus, Cyprus
Abstract
Background and Objectives: Sarco(endo) plasmic reticulum Ca2+-ATPase (SERCA2a) is the ATP-driven pump that translocates Ca2+ from the cytoplasm to the lumen of the sarcoplasmic reticulum. 25% of children and adolescents with type 1 diabetes will develop diastolic dysfunction that results in part from a reduction in the activity of SERCA2a. The present study aimed at investigating and comparing the therapeutic effect of Adipose Mesenchymal Stem Cells (AMSCs) with SERCA2a gene modified AMSCs in diabetes type 1 induced cardiomyopathy of adult male albino rat. Materials and Methods: Twenty-nine adult male albino rats were divided into: Donor group of 2 rats used to obtain AMSCs. Group I (Control group): 6 rats not exposed to diabetes induction. Group II (Diabetic group): 7 rats injected with streptozotocin (STZ) 50mg/Kg once. Group III ( (AMSCs group): 7 rats injected with AMSCs following induction of diabetes. Group IV (SERCA2a Modified AMSCs Group): 7 rats injected with SERCA2a modified AMSCs, following induction of diabetes. All rats were sacrificed 8 weeks from start of experiment. Results: Morphological changes, indicating inflammation and degeneration, were found in the cardiac muscle of diabetic rats and regressed remarkably by AMSCs and SERCA2a modified AMSCs. The regression of morphological changes was confirmed by histological, immunohistochemical, morphometric and serological studies. Conclusion: The therapeutic effect of SERCA2a modified AMSCs in diabetes type 1 cardiomyopathy was more remarkable than that of AMSCs.
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