Effect of Olanzapine on Pancreas of Adult Male Albino Rats and the Possible Role of Granulocyte Colony Stimulating Factor versus Umbelliferon (A Histological, Immunohistochemical and Biochemical Study)

Document Type : Original Article

Authors

1 Department of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt

2 Department of Biochemistry, Faculty of Medicine, Zagazig University, Egypt

Abstract

Introduction: Olanzapine is an atypical antipsychotic prescribed usually for treatment of psychiatric disorders as schizophrenia.
It has been widely documented to induce pancreatitis and type 2 diabetes mellitus. Granulocyte colony stimulating factor (G-CSF)
is an approved drug for treatment of leucopenia and for mobilization of haemopoietic stem cells from bone marrow towards
peripheral circulation. Umbelliferon is a natural plant product that has multiple pharmacological characteristics, including antiinflammatory,
antioxidant, anti-apoptotic and anti-diabetic actions.
Aim of the Work: This research intended to compare the potential therapeutic benefits of umbelliferon and G-CSF on induced
pancreatic injury.
Materials and Methods: We utilized forty-eight adult male albino rats that were subdivided into 4 groups; control group,
olanzapine group, G-CSF treated group and Umbelliferon treated group. All animals were sacrificed at the end of the experiment
and then tissue and serum were gathered for a biochemical analysis. Histological sections from each group were taken and
examined under the light and electron microscopes. Results were subjected to morphometrical and statistical studies.
Results: The histological and biochemical results showed different pathological changes of the pancreatic tissue in olanzapine
group as distorted acini, thickened septa and congested blood vessels. Also elevated serum amylase and oxidative stress
markers as MDA and SOD was shown in the olanzapine group. The GCSF-treated group showed a histological and biochemical
improvement, however the structural and biochemical improvement in the umbelliferon group was more than that shown in the
G-CSF group.
Conclusions: Finally, we concluded that, umbelliferon administration caused a marked ameliorative effect on olanzapine induced
pancreatic injury as a comparable to G-CSF treatment.

Keywords

Journal of Medical Histology
Volume 7, Issue 2
December 2023
Pages 219-240

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