Ebrahem, H. (2019). Modulatory effect of vitamin C on Gentamicin induced nephrotoxicity in male albino Rats (histological effect). Journal of Medical Histology, (), -. doi: 10.21608/jmh.2019.17347.1067
Hana Abd alkader Ebrahem. "Modulatory effect of vitamin C on Gentamicin induced nephrotoxicity in male albino Rats (histological effect)". Journal of Medical Histology, , , 2019, -. doi: 10.21608/jmh.2019.17347.1067
Ebrahem, H. (2019). 'Modulatory effect of vitamin C on Gentamicin induced nephrotoxicity in male albino Rats (histological effect)', Journal of Medical Histology, (), pp. -. doi: 10.21608/jmh.2019.17347.1067
Ebrahem, H. Modulatory effect of vitamin C on Gentamicin induced nephrotoxicity in male albino Rats (histological effect). Journal of Medical Histology, 2019; (): -. doi: 10.21608/jmh.2019.17347.1067
Modulatory effect of vitamin C on Gentamicin induced nephrotoxicity in male albino Rats (histological effect)
Articles in Press, Accepted Manuscript, Available Online from 28 September 2019
Histology department helwan university egypt cairo
Abstract
Abstract
Background: Gentamicin (GM) is an aminoglycoside antibiotic used as bactericidal and effective antibiotic against Gram-negative and limited Gram-positive organisms however nephrotoxicity is one of its side effect. Aim of the work: This study was designed to investigate the effect of vitamin C on the Gentamicin induced histological renal damage. Material and Methods: paraffin sections from rat kidneys were obtained from thirty rats divided in to two groups, control “Group I” and experimental group. The experimental group was divided into groups II and III. Groups II received Gentamicin (70 mg/kg/day I.M) for two weeks, groups III received vitamin C (0.2 mg/kg/day I.P) with Gentamicin for two weeks, Sections stained with Hematoxylin and eosin, PAS, Toluidine blue and Crossman’s stain then examined by light microscopy. Measurements of urea and creatinine in blood for rats in all groups were done followed by statistical analysis of the data. Other specimens were prepared and examined by electron microscopy. Results: L.M of kidney sections showed marked tubular damage especially at PCT in group II. These findings were less manifested by using Vitamin C in group III. These histological changes were confirmed by histochemical stain, image analysis as well as TEM and also proved by measurement of urea and creatinine in blood. The detected morphological, biochemical and functional changes in kidney due to reactive oxygen species that cause nephrotoxicity. Conclusion: The use of vitamin C as antioxidant can improve the renal damage caused by GM.
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