Ibrahim, M., Abdelwahab, S., Mohammed,, H., Ahmed, A. (2022). The Possible Therapeutic Effect of Stem Cells-Conditioned Medium on Statin Induced Animal Model of Myopathy: Biochemical, Histological and Immuno-Histochemical Study. Journal of Medical Histology, 6(1), 60-78. doi: 10.21608/jmh.2023.203584.1114
Manar Ibrahim; Soha Abdelwahab; Hager Mohammed,; Amira Ahmed. "The Possible Therapeutic Effect of Stem Cells-Conditioned Medium on Statin Induced Animal Model of Myopathy: Biochemical, Histological and Immuno-Histochemical Study". Journal of Medical Histology, 6, 1, 2022, 60-78. doi: 10.21608/jmh.2023.203584.1114
Ibrahim, M., Abdelwahab, S., Mohammed,, H., Ahmed, A. (2022). 'The Possible Therapeutic Effect of Stem Cells-Conditioned Medium on Statin Induced Animal Model of Myopathy: Biochemical, Histological and Immuno-Histochemical Study', Journal of Medical Histology, 6(1), pp. 60-78. doi: 10.21608/jmh.2023.203584.1114
Ibrahim, M., Abdelwahab, S., Mohammed,, H., Ahmed, A. The Possible Therapeutic Effect of Stem Cells-Conditioned Medium on Statin Induced Animal Model of Myopathy: Biochemical, Histological and Immuno-Histochemical Study. Journal of Medical Histology, 2022; 6(1): 60-78. doi: 10.21608/jmh.2023.203584.1114
The Possible Therapeutic Effect of Stem Cells-Conditioned Medium on Statin Induced Animal Model of Myopathy: Biochemical, Histological and Immuno-Histochemical Study
1Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Egypt.
2Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Egypt
Abstract
Introduction: Myopathies are diseases with high prevalence rate all over the world. This study aimed to investigate the ability of stem cell conditioned medium (SC-CM) administered by 2 different routes (IM\IV) to repair damaged skeletal muscle. Materials and Methods: This study included 60 adult male albino rats divided equally into five groups: the control group; the myopathy group in which myopathy was induced by administration of ATOR (10 mg/kg/day orally for 4 weeks); IV conditioned medium treated group in which myopathy was induced then the rats were IV injected with 0.5 ml of SC-CM in tail vein, IM conditioned medium treated group the rats were locally injected with 0.5 ml of SC-CM after induction of myopathy, the untreated myopathy group was left without treatment for 4 weeks. Blood samples and muscular specimens from all groups were collected for biochemical and histological studies. Results: The myopathy group showed marked muscle degeneration, with inflammatory cells infiltration. Compared with control, there was significant decrease in the area% of PAS and significant increase in mean creatinine kinase and Myoglobin levels , in area% of collagen and caspase3 positive reaction and the number of myogenin positive cells. Four weeks after SC-CM injection (IM\IV), there was marked improvement in histological architecture of the skeletal muscle as there was significant decrease in mean creatinine kinase and Myoglobin levels and in area% of collagen and caspase positive cells and significant increase in area% of PAS and number of myogenin positive cells and the improvement was more obvious in IV injected group. Conclusion: SC-CM injection may be of great help in the management of critical cases of myopathy especially by IV route.
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