The Possible Therapeutic Effect of Stem Cells-Conditioned Medium on Statin Induced Animal Model of Myopathy: Biochemical, Histological and Immuno-Histochemical Study

Document Type : Original Article

Authors

1 Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Egypt.

2 Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Egypt

Abstract

Introduction: Myopathies are diseases with high prevalence rate all over the world. This study aimed to investigate the ability
of stem cell conditioned medium (SC-CM) administered by 2 different routes (IM\IV) to repair damaged skeletal muscle.
Materials and Methods: This study included 60 adult male albino rats divided equally into five groups: the control group;
the myopathy group in which myopathy was induced by administration of ATOR (10 mg/kg/day orally for 4 weeks); IV
conditioned medium treated group in which myopathy was induced then the rats were IV injected with 0.5 ml of SC-CM in tail
vein, IM conditioned medium treated group the rats were locally injected with 0.5 ml of SC-CM after induction of myopathy,
the untreated myopathy group was left without treatment for 4 weeks. Blood samples and muscular specimens from all groups
were collected for biochemical and histological studies.
Results: The myopathy group showed marked muscle degeneration, with inflammatory cells infiltration. Compared with
control, there was significant decrease in the area% of PAS and significant increase in mean creatinine kinase and Myoglobin
levels , in area% of collagen and caspase3 positive reaction and the number of myogenin positive cells. Four weeks after
SC-CM injection (IM\IV), there was marked improvement in histological architecture of the skeletal muscle as there was
significant decrease in mean creatinine kinase and Myoglobin levels and in area% of collagen and caspase positive cells and
significant increase in area% of PAS and number of myogenin positive cells and the improvement was more obvious in IV
injected group.
Conclusion: SC-CM injection may be of great help in the management of critical cases of myopathy especially by IV route.

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