Possible Protective Effects of Quercetin versus Quercetin Loaded Chitosan Nanoparticles on Zinc Sulfate Induced Olfactory Mucosa and bulb Injury in Adult Male Albino Rats; Behavioral, Biochemical, Histological and immunohistochemical study

Document Type : Original Article

Authors

1 Department of Histology and Cell BiologyوFaculty of Medicine, Minia University, Minia, Egypt

2 Department of Anatomy and Embryology, Faculty of Medicine, Minia University, Minia, Egypt

3 Department of Medical Pharmacology, Faculty of Medicine, Minia University, Minia, Egypt

4 Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Minia, Egypt

Abstract

Background: Anosmia is a common annoying problem that affects quality of life. Nanoparticles act as nanocarriers that
deliver potent drug payloads at a controlled rate at the target site.
Purpose: This current work aimed to evaluate the potential preventive benefits of Quercetin (QU) versus Quercetin-loaded
chitosan nanoparticles (QU-CSNPs) on an experimental rat model of anosmia and shed insight on its protective mechanisms.
Methods: Forty rats were randomly divided into four groups, the Control group, the Zinc sulfate (ZnS) group, the ZnS+QU
group, and the ZnS+QU-CSNPs group. Behavioral assessment was carried out. Olfactory mucosae and bulbs were collected
for biochemical, histological, immunohistochemical, and morphometric analysis.
Results: The ZnS group showed behavioral alternation, decreased glutathione levels, increased malonaldehyde, and tumor
necrosis factor-α levels. Olfactory mucosa and bulb showed histological alterations, as well as a significant increase in COX-
2 immune-expression and decrease in PCNA and SOX2 expression. The ZnS+QU-CSNPs group showed amelioration in
previously mentioned parameters and histological alternation than the ZnS+QU group.
Conclusions: QU-CSNPs has prophylactic effects in an anosmia model than QU via its anti-inflammatory and antioxidant
effects. This could pave the way for additional studies to investigate further mechanisms underlying the potential beneficial
effects of QU-CSNPs and bring up a new line for anosmia therapy.

Keywords

Journal of Medical Histology
Volume 7, Issue 2
December 2023
Pages 185-218

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