Dawood, A., Hareedy, H. (2019). Differential effect of high fat diet (HFD) on the cardiac muscle of adult and aged female mice and the possible protective role of artichoke treatment: Histomorphometric and ultrastructural study. Journal of Medical Histology, 3(1), 36-54. doi: 10.21608/jmh.2019.11528.1053
Asmaa Dawood; Hemmat Hareedy. "Differential effect of high fat diet (HFD) on the cardiac muscle of adult and aged female mice and the possible protective role of artichoke treatment: Histomorphometric and ultrastructural study". Journal of Medical Histology, 3, 1, 2019, 36-54. doi: 10.21608/jmh.2019.11528.1053
Dawood, A., Hareedy, H. (2019). 'Differential effect of high fat diet (HFD) on the cardiac muscle of adult and aged female mice and the possible protective role of artichoke treatment: Histomorphometric and ultrastructural study', Journal of Medical Histology, 3(1), pp. 36-54. doi: 10.21608/jmh.2019.11528.1053
Dawood, A., Hareedy, H. Differential effect of high fat diet (HFD) on the cardiac muscle of adult and aged female mice and the possible protective role of artichoke treatment: Histomorphometric and ultrastructural study. Journal of Medical Histology, 2019; 3(1): 36-54. doi: 10.21608/jmh.2019.11528.1053
Differential effect of high fat diet (HFD) on the cardiac muscle of adult and aged female mice and the possible protective role of artichoke treatment: Histomorphometric and ultrastructural study
1Department of Histology, Faculty of Medicine, Assiut University, Assiut, Egypt, Department of Biomedical Science, Faculty of Medicine, King Faisal University, Alhasa, Saudi Arabia,
2Department of Basic Medicine, Majma'ah University, Saudi Arabia.
Abstract
Background: Coexistence of hyperlipidemia and aging is a highly serious condition that massively deteriorates cardiac function. Artichoke is a well-known antioxidant commonly used in long-lived countries diet. Recent researches showed its hypolipidemic and antiaging effects. Objective: This work aimed to investigate the histopathological effects of high fat diet (HFD) on adult mice cardiac muscle, to address and compare the extent of myocardial damage in aged mice and to assess beneficial effects of artichoke in mitigating these effects. Materials and Methods: A total of 42 adult (Group I) and aged (Group II) female mice (3 and 26 months old respectively) were divided into six equal subgroups (7 per each); Groups Ia and IIa: adult and aged control, Groups Ib and IIb: adult and aged HFD groups were fed diet enriched with 1.5% cholesterol and 10% lard oil for 6 weeks. Groups Ic and IIc: adult and aged artichoke treated group, were received HFD for 6 weeks as HFD groups concomitant with artichoke extract at a dose of 1.5 gm/kg once daily via gastric tube. Results: Adult HFD laden cardiac myocytes showed loss of cross striations, inflammatory cell infiltrate, increase collagen content, myofiber focal degeneration,disruption of myofibrils, sarcomeres and T tubule system, lipid vacuolation, mitochondrial damage and nuclear pyknosis. These effects were the worst in aged HFD fed animals. Artichoke markedly improved these changes in both adult and aged mice. Conclusion: combination of HFD and aging caused more extensive insults on cardiac myocytes than each factor alone. Artichoke is highly efficient in ameliorating these insults.
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